Cell Harvesting Solutions by Sartorius
Sartoclear Dynamics® Lab — Convenient Mammalian Cell Culture Harvesting
Sartoclear Dynamics® Lab kits are designed for rapid harvesting 15 mL to 1,000 mL volumes of cell cultures in the lab, enabling clarification and sterile filtration to be performed in one step. These kits simplify the process by fully eliminating the centrifugation step otherwise needed for clarification. As a result, up to 1,000 mL cell cultures can be efficiently clarified and sterilized in minutes – quickly and easily.
Product Portfolio
Rapid Harvest and Concentration of AAVs with Sartoclear Dynamics®️ Lab and Vivaflow®
This video provides a detailed walkthrough of the purification process, starting from cell lysis all the way to obtaining a concentrated retentate. It highlights the use of a clarification step employing Sartoclear Dynamics®️ Lab to eliminate larger contaminants such as cells and cell debris. Following this, an ultrafiltration step using Vivaflow®️ is demonstrated to concentrate the AAV particles and further cleanse the preparation of smaller impurities.
Join us to explore the intricacies of AAV purification and enhance your understanding of this vital component of gene therapy development.
Sartoclear Dynamics® Lab Filtration Kits
Sartoclear Dynamics Lab clarifies high-density mammalian cell cultures faster and more efficiently than other processes.
Watch this comparison video.
Sartoclear Dynamics® Lab V Kits
Clarification and sterile filtration of up to 1 L mammalian cell culture combining a filter aid for clarification and a vacuum filtration unit for sterile filtration.
- Sterilized pouches of filter aid and vacuum filtration units
- 14 kits to cover a range of sample volumes up to 1,000 mL with cell densities up to 20 x 106 cells/mL
Sartolab® Multistation for Hands-Free Filtration of Small Volumes
For simultaneous filtration of up to 6 x 50 mL samples without the need for installation of extra connectors and time-consuming stabilization of filter units.
- Hands-free filtration
- One single vacuum source enables simultaneous filtration of up to 6 samples
- Time-saving - no installation time for each filter unit before use
Sartoclear Dynamics® Lab P15 Kit
Convenient, ready-to-use-kit combines a 20 mL syringe pre-filled with a filter aid for clarification with a 0.2 um polyethersulfone filter for sterile filtration.
- One filtration kit designed for all cell densities
Sartoclear Dynamics® Lab and Multistation in Action
LifeArc Testimonial
The team at LifeArc, a medical research charity based in the UK, is using the Sartoclear Dynamics®️ Lab and Sartolab®️ Multistation to clarify high-density cell culture media in minutes, creating a workflow that’s more efficient and productive.
"Processing mammalian cell culture fluid can be particularly challenging, as in the lab we are often driven by the need to perform this task quickly with minimum loss in the quantity of cell culture fluid obtained. Standard vacuum filtration isn’t always the ideal solution due to limitations in the sophistication of the equipment itself. It often requires additional supervision and can be quite time-consuming to undertake, particularly when there are several samples involved.
The Multistation has significantly helped our lab. In addition to the six-sample capacity, the independently controlled multi-port design has provided operators with the flexibility to perform additional tasks in parallel, thereby delivering greater efficiency overall in the lab with improved cell culture fluid harvesting."
- Richard Brown, BSc, Scientist at LifeArc
Testimonial
Sirion Biotech GmbH
The team at Sirion Biotech GmbH, a leading viral vector manufacturer based in Germany, is using the Sartoclear Dynamics®️ Lab and Multistation to achieve clarification within minutes while simultaneously filtering up to 6 L, significantly accelerating their upstream processes.
"As a leading viral-vector manufacturer, we strive to produce the highest quality rAAVs in the shortest possible time. Isolating rAAVs from crude cell-lysates typically requires centrifugation and/or PEG precipitation – steps which are time consuming, labor intensive and generate a lot of plastic waste. The single-use Sartoclear Dynamics® Lab Filter kits has enabled us to streamline our upstream processes. The crude-lysates are directly filtered over Sartoclear Dynamics® Lab Filter kits. Clarification is achieved within a few minutes and the Multistation set-up allows us to simultaneously filter up to 6 L. The rAAV-recoveries using Sartoclear Dynamics® Lab products are remarkably high, surpassing what we obtain using in-depth filtration. Moreover, lysates clarified using Sartoclear Dynamics® Lab are instantly ready for our downstream processes including affinity capture by FPLC. Sartoclear Dynamics® Lab, along with the Multistation, have greatly improved our workflow."
- Kimchi Strasser, PhD., Team Lead for Process Development at Sirion Biotech GmbH (a Perkin Elmer company)
