Take your RNA research to the next level with QIAGEN LNA tools!
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Download the flyer! Experience truly exceptional RNA research with QIAGEN's next-generation, LNA®-enhanced tools. LNA (Locked Nucleic Acid) oligos bind with much higher affinity and specificity to RNA targets than standard DNA and RNA oligos – This enables specific and sensitive detection of small RNAs and discrimination between highly similar sequences.
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Take your RNA research to the next level with QIAGEN LNA tools!
- 1. Take your RNA research to the next level with QIAGEN LNA tools Experience truly exceptional RNA research with our next-generation, LNA® -enhanced tools. LNA (locked nucleic acids) oligos bind with much higher affinity and specificity to RNA targets than standard DNA and RNA oligos – This enables specific and sensitive detection of small RNAs and discrimination between highly similar sequences. We have merged QIAGEN’s cutting edge technologies with Exiqon’s 20 years of experience in LNA oligo design to enhance your qPCR assays, in situ hybridization and functional analysis in cells and animals, miRNA and RNA sequencing and much more! Sample to Insight
- 2. 2 Take your RNA research to the next level with QIAGEN LNA tools 05/2018 Figure 2. LNA Tm normalization. The affinity of traditional, full length, microRNA inhibitors without LNA is highly influenced by GC-content, with Tm values spanning >40˚C. The Tm of miRCURY LNA microRNA inhibitors span just 10˚C around an optimal high temperature, ensuring uniform, higher potency. 30 40 50 RNA Tm miRNA GC content (%) 60 70 80 Traditional DNA inhibitors LNA inhibitors • Exceptional sensitivity: Detect RNA expressed at very low levels or in complex samples i.e. liquid biopsies • Superior specificity: Accurately discriminate between closely related sequences – Achieve even single-nucleotide mismatch discrimination • Higher affinity: Design more potent and sensitive oligos. Enables the design of shorter probes or primers to target specific isoforms and helps overcome the difficulties in studying very short sequences i.e. microRNAs • Enhanced thermal stability: Achieve uniform detection regardless of GC content with the freedom to place probes and primers where needed • Resistant to exo- and endonucleases: Benefit from long lasting stability in vitro and in vivo LNA (Locked Nucleic Acids) are a class of high-affinity RNA analogs in which the ribose ring is “locked” in a sterical conformation which has the unique property of greatly enhancing the strength of Watson-Crick base-pairing. The power of LNA technology LNA substitutions increase oligo melting temperature, enabling Tm adjustment and normalization, regardless of oligo length or GC content. LNA is optimal for designing short, high-affinity PCR primers, detection probes and antisense oligonucleotides. Figure 1. Substituting LNA for DNA gives higher melting temperatures. Unmatched hybridization affinity - regardless of GC content
- 3. Take your RNA research to the next level with QIAGEN LNA tools 05/2018 3 LNA increases the Tm difference between perfectly matched and mismatched targets – giving an increased signal-to-noise ratio. This broadens the detection window and boots assay specificity. Even closely related sequences that differ by as little as one nucleotide can be accurately discriminated, in ISH studies, qPCR profiling and silencing. Figure 4. Superior signal-to-noise ratios. The signal obtained from perfectly matched LNA oligos (100%) is compared to signal from LNA oligos with a single nucleotide mismatch. Exceptional specificity – achieve single–nucleotide mismatch discrimination Due to their short length, high-affinity, LNA antisense oligonucleotides are taken up naturally by cells. Highly potent once inside cells and resistant to enzymatic degradation, LNA antisense oligos effectively silence RNA in a broad range of animal tissues – making them the tool of choice for revealing RNA functions and evaluating their therapeutic potential. Figure 5. Efficient in vivo knockdown with LNA GapmeRs in a broad range of tissues. The Antisense LNA GapmeR for knockdown of Malat 1 in mice was injected subcutaneously over a period of 5 weeks. Tissue samples from the mice were collected 2 days after the final LNA GapmeR administration. The results show highly efficient knockdown of Malat1 in a broad range of different tissues. MALAT1 RNA level % (relative to PBS control) 120 100 80 60 40 20 0 2 days after last dose of MALAT1 LNA GapmeR Liver Jejunum Ovaries Kidney Heart Fatpad Skeletal muscle Skin Spleen Lung Brain Effective RNA silencing - with the robustness and stability for animal models Figure 3. Ultra-sensitive and specific miRNA detection with LNA. Detection of SSA4 RNA in ∆rip1 fixed yeast cells using a single-labeled Cy3 Custom LNA mRNA Detection Probe (left) or a single-labeled Cy3 DNA oligonucleotide (right) of the same length. LNA DNA THJ202 LNA inclusion in oligos vastly increases binding affinity and allows the design of highly sensitive qPCR and ISH detection assays. Detect more transcripts – even miRNA and lncRNA at low expression levels. Use less sample material or complex samples with low RNA content. Unsurpassed sensitivity – see the difference LNA technology makes
- 4. Ordering www.qiagen.com/shop Technical Support support.qiagen.com Website www.qiagen.com QIAGEN LNA-enhanced RNA analysis products Product groups Description Sequencing QIAseq™ miRNA Library Kit LNA-enhanced, gel-free miRNA sequencing library prep from as little as 1 ng of total RNA with integrated Unique Molecular Indices (UMIs) for accuracy in quantification QIAseq UPX 3’ Transcriptome Kit and Targeted RNA panels Ultraplex (UPX) library preparation for high-throughput gene expression analysis from ultralow amounts of RNA or cells, with LNA-enhanced chemistry and UMIs QIAseq miRNA Library QC PCR Panels and Assays RNA sample quality evaluation prior to miRNA/small RNA NGS library preparation and for assessing NGS performance post-sequencing qPCR miRCURY™ LNA miRNA PCR System Unique miRNA qPCR system includes a universal cDNA reaction for all miRNAs and the sensitivity and specificity of LNA-enhanced miRNA primers. Detection miRCURY LNA miRNA Probes and Custom LNA mRNA Detection Probes For ultra-sensitive and specific miRNA, mRNA or lncRNA detection by in situ hybridization (ISH) or Northern blotting Functionalanalysis miRCURY LNA miRNA Inhibitors, Mimics and Target Site Blockers Exceptionally potent mimics, inhibitors and target site blockers, enable functional analysis of specific miRNAs and determination of gene regulation roles, even in difficult-to-transfect cell lines and in vivo Antisense LNA GapmeRs Antisense LNA GapmeRs are highly potent, single-stranded antisense oligonucleotides for silencing lncRNA and mRNA in cell cultures and in vivo, in animal models Custom LNA-enhanced Oligonucleotides Design your own custom LNA-enhanced oligonucleotides with our online QIAGEN LNA design tools or let QIAGEN's LNA experts design them for you. We offer custom oligo synthesis with a wide variety of possible modifications, labels, synthesis scales and purification methods. Proper incorporation of LNA in a sequence can produce an oligo of dramatically increased affinity, specificity and allelic discrimination potential. Benefit from our 20 years of LNA-oligo design experience – We will find the right design for your experimental purpose and target of interest. Learn more at www.qiagen.com/LNA For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor. Trademarks: QIAGEN® , Sample to Insight® , LNA® , QIAseq™ , miRCURY™ (QIAGEN Group). PROM-12093-001 © 2018 QIAGEN, all rights reserved. 1112423 05/18